Protective effects of hawthorn (Crataegus pinnatifida) polyphenol extract against UVB ‐induced skin damage by modulating the p53 mitochondrial pathway in vitro and in vivo

This study investigated the effect of a hawthorn polyphenol extract (HPE) on ultraviolet B (UVB) ‐induced damage in HaCaT cells and mice. High‐performance liquid chromatography/electrospray ionization tandem mass spectrometry was used to analyze the phenolic composition of HPE. The protective effects of HPE and its main components were compared in HaCaT cells. An enzyme‐linked immunosorbe nt assay was used to detect DNA damage (8‐hydroxydeoxyguanosine levels). Flow cytometry and western blotting were used to measure the extent of apoptosis and the levels of apoptosis‐related proteins, respectively. Treatment with HPE or its polyphenol components inhibited the UVB‐induced damage by removing an excess of reactive oxygen species (ROS), reducing DNA damage and p53 activation, regulating the protein expression of B‐cell lymphoma 2 family members toward antiapoptotic ratios, and reducing caspase activation. Similar effects were observed in a UVB‐irradiated mouse skin, as de tected using terminal deoxynucleotidyl transferase dUTP nick‐end labeling, immunohistochemistry, and western blotting assays. These results suggest that HPE can be used as a natural dietary supplement for the prevention and treatment of UVB radiation‐induced skin damage.Practical applicationsHawthorn (Crataegus pinnatifida) shows antioxidant, anti ‐inflammatory, and lipid‐lowering effects. As natural, healthy, and effective additives, HPEs have been widely used in food and health products...
Source: Journal of Food Biochemistry - Category: Food Science Authors: Tags: FULL ARTICLE Source Type: research