Venoarterial Extracorporeal Membrane Oxygenation Increased Immune Function of Spleen and Decreased Reactive Oxygen Species During Post ‐Resuscitation

AbstractWe aimed to investigate the effect of venoarterial extracorporeal membrane oxygenation (VA ‐ECMO) on immune function of the spleen and reactive oxygen species (ROS) during post‐resuscitation in a porcine model. After 8 min of untreated ventricular fibrillation and 6 min of basic life support, pigs were randomized into two groups: Group 1 received VA‐ECMO and Group 2 received conve ntional cardiopulmonary resuscitation. After successful return of spontaneous circulation, the hemodynamic status was determined and blood samples were collected at 0, 1, 2, 4, and 6 h. Surviving pigs were euthanized 6 h after return of spontaneous circulation, their spleens were harvested and the T‐cells were separated. Then, we investigated immune function parameters of the spleen and ROS levels. VA‐ECMO increased the return of spontaneous circulation and 6 h survival rate after return of spontaneous circulation. Compared with the conventional cardiopulmonary resuscitation group, the VA‐ECMO group showed increased superoxide dismutase and decreased malondialdehyde and ROS levels. Furthermore, VA‐ECMO was associated with a high rate of CD4+ and CD4+/CD8+, high levels of interleukin 2, interferon γ, and interferon γ/interleukin 4, as well as high proliferation of lymphocytes . The apoptotic rate of T‐cells was lower in the VA‐ECMO group than it was in the conventional cardiopulmonary resuscitation group. VA‐ECMO increased immune function of spleen and decreased ROS...
Source: Artificial Organs - Category: Transplant Surgery Authors: Tags: Main Text Article Source Type: research