Real time monitoring and quantification of uptake carbon nanodots in eukaryotic cells

• Carbon nanodots have linear dependence between their concentration and emission intensity in aqueous solution and cytoplasm of living cells.• The relative emission intensity and concentration values of carbon nanodots were quantified by confocal microscope in Tobacco BY‐2 plant cells. AbstractThe real time monitoring and quantification of the concentration of highly fluorescent nitrogen ‐doped carbon nanodots (C‐dots) in eukaryotic Tobacco bright yellow‐2 (BY‐2) plant cells was investigated by fluorescence and confocal microscopy. The quantitative measurement of their fluorescent emission intensity was possible because of the high photo‐resistance, good water solubility a nd the absence of fading effect of the nanoparticles, which is frequent occurred problem of the conventional organic dyes. The microscopic analysis revealed that C‐dots entered generally into the cells through endocytosis and caused negligible cytotoxicity. The multicolor cellular imaging of label ed Tobacco BY‐2 demonstrates that the cells were in good health conditions and any blinking artifacts were not observed. The quantification of fluorescence emission intensity was carried out in the intracellular regions where the relationship between the C‐dots concentration and relative emissio n was linear. Based on a control experiment with fluorescence liposomes with known dependence between C‐dots concentration and emission, we were able to determine the amount of accumulated nanopart...
Source: Microscopy Research and Technique - Category: Laboratory Medicine Authors: Tags: RESEARCH ARTICLE Source Type: research