Isolation, characterization and antioxidant activity of hyaluronic acid from marine bivalve mollusc Amussium pleuronectus (Linnaeus, 1758)

Publication date: July 2013 Source:Bioactive Carbohydrates and Dietary Fibre, Volume 2, Issue 1 Author(s): Shankar Kanchana , Muthuvel Arumugam , Sadhasivam Giji , Thangavel Balasubramanian The hyaluronic acid (HA) was isolated from bivalve mollusk Amussium pleuronectus and its antioxidant potential was evaluated. HA was separated from glycosaminoglycans mixture using ion-exchange chromatography (DEAE-cellulose). The net yield of HA was found to be 4.2mg/g. The presence of HA was confirmed by agarose gel electrophoresis and further analyzed by a standard calorimetric method using Stains All. The disaccharide composition revealed the presence of uronic acid (47.7%) and N-acetyl glucosamine (35.1%). The structure of HA was characterized through FT-IR and 1H NMR spectroscopy. At 1mg/ml, the scavenging ability of isolated HA towards ABTS, DPPH and hydroxyl radicals was 71.35, 54.42 and 63.42%, respectively. The isolated HA could be used as a potent antioxidant agent. Graphical abstract
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research