Structural characterization of β glucans isolated from Agaricus blazei Murill using NMR and FTIR spectroscopy

Publication date: October 2013 Source:Bioactive Carbohydrates and Dietary Fibre, Volume 2, Issue 2 Author(s): Maria Leônia C. Gonzaga , Thiago M.F. Menezes , José Roberto R. de Souza , Nágila M.P.S. Ricardo , Sandra de A. Soares Many interesting biological activities have been reported related to the presence of β-glucans from different species of mushrooms. In this work, β-glucans were isolated from the polysaccharide of the fruiting bodies of Agaricus blazei Murill known as Agaricus brasiliensis. β-Glucans were obtained with a specific procedure using thymol. The isolated material was characterized by FTIR assignments which indicate the presence of polysaccharides and some amount of protein in the obtained precipitate. The presence of β-glucans was evidenced by recording the band around 890cm−1 relative to the deformation of that bond. 1H and 13C NMR as well as two-dimensional COSY and HMQC spectra were used for characterization of the β-glucans which showed that the isolated material is constituted mainly by β-(1→6) glucans. Graphical abstract

Source: Bioactive Carbohydrates and Dietary Fibre - October 12, 2014 Category: Food Science Source Type: research