Bioactivity of sulfated polysaccharides from the edible red seaweed Mastocarpus stellatus

Publication date: January 2014 Source:Bioactive Carbohydrates and Dietary Fibre, Volume 3, Issue 1 Author(s): Eva Gómez-Ordóñez , Antonio Jiménez-Escrig , Pilar Rupérez Carrageenans are typical sulfated-galactans from red seaweeds. Their main biological properties as antioxidants and anticoagulants were studied in vitro in Mastocarpus stellatus. On sequential extraction with cold-water (F1), hot-water (F2), acid (F3) and alkali (F4), four soluble polysaccharide fractions plus an insoluble cellulose residue (F5) were obtained. Composition was assessed by gas–liquid chromatography, ion chromatography and Fourier Transform Infrared (FTIR-ATR) spectroscopy. Multifunctional antioxidant capacity was assayed by FRAP- reducing power, ABTS- and photochemiluminiscence (PCL)- radical scavenging methods. Heparin-like anticoagulant capacity was studied by intrinsic-APTT and extrinsic-PT pathways. Recovery of F1–F4 yielded almost 60%, which main components were: neutral sugar (21.2–34.7g/100g), sulfate (0.6–3.9g/100g), protein (0.1–3.4g/100g) and uronic acid (0.9–2.7g/100g). Galactose (70.2–95.2mol/100g) was the predominant sugar in F1–F4 and sulfate their main inorganic anion (75–80% of total). F1–F3 contained the bulk of carrageenans. Degree of sulfation ranged from 0.22 (F1) to 0.03 (F3), respectively. Typical absorption bands of kappa-/iota-carrageenans at 930, 845 and 805cm−1 assigned respectively to 3,6-anhydro-galactose, galactose-4-sulfate and 3,6-...
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research