Inflammatory markers in Huntington's disease plasma—A robust nanoLC–MRM-MS assay development

We report a robust and sensitive quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing. The assay has a dynamic range with 4 orders of magnitude and the LOQs are in the attomolar range. We used plasma from Huntington's disease gene carriers and healthy controls to compare our MRM method with antibody based methods. Importantly, we found a good agreement between assays for the measurement of C-reactive protein, in contrast to complement component 3 and complement factor H. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research