Characterisation of potential novel allergens in the fish parasite Anisakis simplex

Publication date: September 2014 Source:EuPA Open Proteomics, Volume 4 Author(s): Christiane Kruse Fæste , Karen R. Jonscher , Maaike M.W.B. Dooper , Wolfgang Egge-Jacobsen , Anders Moen , Alvaro Daschner , Eliann Egaas , Uwe Christians The parasitic nematode Anisakis simplex occurs in fish stocks in temperate seas. A. simplex contamination of fish products is unsavoury and a health concern considering human infection with live larvae (anisakiasis) and allergic reactions to anisakid proteins in seafood. Protein extracts of A. simplex produce complex band patterns in gel electrophoresis and IgE-immunostaining. In the present study potential allergens have been characterised using sera from A. simplex-sensitised patients and proteome data obtained by mass spectrometry. A. simplex proteins were homologous to allergens in other nematodes, insects, and shellfish indicating cross-reactivity. Characteristic marker peptides for relevant A. simplex proteins were described. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research