Proteome profiling illustrated by a large-scale fed-batch fermentation of Penicillium chrysogenum

Publication date: September 2014 Source:EuPA Open Proteomics, Volume 4 Author(s): Michaela Helmel , Andreas Posch , Christoph Herwig , Günter Allmaier , Martina Marchetti-Deschmann Filamentous fungi are employed for the large-scale production of value-added products, including organic acids, enzymes, and antibiotics and bioprocess characterization is essential for production optimization but relies on empiricism-based strategies. Protein expression profiles in an industrial scale, 180h fed-batch fermentation of Penicillium chrysogenum are presented. The biomass of P. chrysogenum, as well as the specific penicillin V production rate and fungal morphology were monitored during fermentation to be compared with obtained protein profiles. Our results demonstrate a correlation between proteomics data and biomass concentration, morphological changes, and penicillin production. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research