Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach

Publication date: September 2014 Source:EuPA Open Proteomics, Volume 4 Author(s): Arivusudar Marimuthu , Tai-Chung Huang , Lakshmi Dhevi N. Selvan , Santosh Renuse , Raja Sekhar Nirujogi , Praveen Kumar , Sneha M. Pinto , Sudha Rajagopalan , Akhilesh Pandey , H.C. Harsha , Aditi Chatterjee miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research