Comparison of label-free quantification methods for the determination of protein complexes subunits stoichiometry

Publication date: September 2014 Source:EuPA Open Proteomics, Volume 4 Author(s): Bertrand Fabre , Thomas Lambour , David Bouyssié , Thomas Menneteau , Bernard Monsarrat , Odile Burlet-Schiltz , Marie-Pierre Bousquet-Dubouch Protein complexes are the main molecular machines that support all major cellular pathways and their in-depth characterization are essential to understand their functions. Determining the stoichiometry of the different subunits of a protein complex still remains challenging. Recently, many label-free quantitative proteomic approaches have been developed to study the composition of protein complexes. It is therefore of great interest to evaluate these different methods in a stoichiometry oriented objective. Here we compare the ability of four absolute quantitative label-free methods currently used in proteomic studies to determine the stoichiometry of a well-characterized protein complex, the 26S proteasome. Graphical abstract

Source: EuPA Open Proteomics - October 13, 2014 Category: Bioinformatics Source Type: research