Repeated isoflurane in adult male mice leads to acute and persistent motor decrements with long ‐term modifications in corpus callosum microstructural integrity

We report that repeated isoflurance exposure can lead to persistent motor impairments and long ‐term alteration in white matter microstructural organization in mice. AbstractIsoflurane is a commonly used inhalational anesthetic, clinically and in animal experimental studies. Although it has been reported as safe, recent findings suggest that despite widespread use, isoflurane ‐induced inhalational anesthesia can lead to various pathophysiological and cognitive alterations. Therefore, we aimed to investigate the long‐term behavioral and white matter consequences of repeated isoflurane exposure. Twenty 3‐month‐old C57BL/6J male mice received one exposure of isoflur ane for 40 min or 2 exposures to isoflurane separated by 3 days. Behavioral paradigms (open field, balance beam, foot fault, rotarod, elevated zero maze, tail suspension, water maze, and social recognition tests) were administered at 1, 3, 5, 7, and 90 days post exposure. Animals exposed to repea ted isoflurane showed significant motor deficits on the balance beam and increased anxiety‐like behavior. Animals exposed to single isoflurane showed impaired performance on the foot fault test. Diffusion tensor imaging showed that repeated isoflurane exposure led to long‐term disruption of wate r diffusivity in corpus callosum (CC) white matter. Furthermore, 2‐D structure‐tensor analysis from stained brain sections showed differences in the microstructural organization of CC white matter in mice with si...
Source: Journal of Neuroscience Research - Category: Neuroscience Authors: Tags: RESEARCH ARTICLE Source Type: research