Protective Effect of Grape Seed Procyanidins against H2O2 ‐Induced Oxidative Stress in PC‐12 Neuroblastoma Cells: Structure–Activity Relationships

AbstractTo establish a structure –activity relationship for procyanidins, we verified the cyto‐protective effect of 13 grape seed procyanidins, ranging from monomers to trimers against H2O2‐induced oxidative stress in PC‐12 neuroblastoma cells. Our study demonstrated some procyanidins were able to significantly protect PC‐12 cells from the H2O2‐induced cytotoxicity suggesting they possess neuroprotective effects against oxidative stress. Procyanidins’ protective effects against oxidative stress mainly depended on their polymerization degree in addition to their structural features. A positive correlation was found between procyanidins ’ polymerization degree and the protective effect against oxidative stress in PC‐12 cells. The presence of 3‐ or 3’‐galloylated groups in the C‐ring of procyanidin molecules significantly increased their protective activity as well. These results demonstrated that galloylated high‐mole cular‐mass procyanidins would be of more interesting as promising antioxidant natural compounds. This work for the first time demonstrated the structure–activity relationships of 13 procyanidins’ antioxidative stress activity, which could have a significant impact on future development of proc yanidins for healthy food products or drugs to treat disease such as neurodegenerative disorders.Practical ApplicationThis work evaluated the protective effect of procyanidins against oxidative stress in PC ‐12 neuroblastoma cells and esta...
Source: Journal of Food Science - Category: Food Science Authors: Tags: Health, Nutrition, & Food Source Type: research