Phospholipase C-β1 interacts with cyclin E in adipose- derived stem cells osteogenic differentiation

Publication date: Available online 5 November 2018Source: Advances in Biological RegulationAuthor(s): Giulia Ramazzotti, Roberta Fiume, Francesca Chiarini, Gabriele Campana, Stefano Ratti, Anna Maria Billi, Lucia Manzoli, Matilde Y. Follo, Pann-Gill Suh, James McCubrey, Lucio Cocco, Irene FaenzaAbstractAdipose-derived stem cells (ADSCs) are multipotent mesenchymal stem cells that have the ability to differentiate into several cell types, including chondrocytes, osteoblasts, adipocytes, and neural cells. Given their easy accessibility and abundance, they became an attractive source of mesenchymal stem cells, as well as candidates for developing new treatments for reconstructive medicine and tissue engineering. Our study identifies a new signaling pathway that promotes ADSCs osteogenic differentiation and links the lipid signaling enzyme phospholipase C (PLC)-β1 to the expression of the cell cycle protein cyclin E. During osteogenic differentiation PLC-β1 expression varies concomitantly with cyclin E expression and we show that the two proteins interact. These findings contribute to clarify the pathways involved in osteogenic differentiation and provide evidence to develop therapeutic strategies for bone regeneration.Graphical abstract
Source: Advances in Biological Regulation - Category: Biology Source Type: research