Pro‐ and anti‐inflammatory cytokine gene single‐nucleotide polymorphisms in inflammatory bowel disease

Summary Anti‐inflammatory cytokines have an important role in disease, tumour and transplant processes. Alterations in the regulation of several cytokines have been implicated in a variety of inflammatory disorders, including IBD (inflammatory bowel disease) [Crohn′s disease (CD) and ulcerative colitis (UC)]. Cytokine polymorphisms are also known to affect the level of gene expression. Thus, the aim of this study was to determine the relationship between cytokine polymorphisms and the IBD pathologies in a Spanish population. Polymorphisms analysis was performed using PCR‐SSOP using a microbeads luminex assay. The following polymorphisms were determined: TNFα [−238G/A (rs361525) and −308G/A (rs1800629)], IFNγ [+874A/T (rs62559044)], TGFβ [+869C/T (rs1982073) and +915G/C (rs1800471)], IL10 [−1082A/A (rs1800896), −592A/C (rs1800872), −819C/T (rs1800871)], IL6 [−174C/G (rs1800795)], IL12p40 [3′UTR −1188A/C (rs3212227)], IL1α [−889C/T (rs1800587)], IL1β [−511C/T (rs1143634) and +3962C/T (rs1143633)], IL1R [Pst‐1 1970C/T] and IL1RA [Mspa‐1 11100C/T]. No statistical differences in TNFα, IFNγ, TGFβ, IL10, IL6, IL1α, IL1β, IL1R and IL1Ra genotypes and allele distributions between the IBD groups and healthy controls were found. However, we observed significant differences in the 3′UTR −1188A/C polymorphism of IL12p40. So −1188A allele was increased in patients with UC and the −1188C allele (high IL12p40 production) was increased in patien...
Source: International Journal of Immunogenetics - Category: Genetics & Stem Cells Authors: Tags: Original Article Source Type: research