Transactivation of Large Ribozymes

The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure–function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.

Source: Springer protocols feed by Genetics/Genomics - October 30, 2014 Category: Genetics & Stem Cells Source Type: news