Effect of using two cryopreservation methods on viability and fertility of frozen stallion sperm

This study evaluated the sperm kinetics, plasma membrane integrity and fertility rates of mares inseminated with cryopreserved stallion semen subjected to different freezing methods. For this, four ejaculates from 5 stallions were collected and frozen in conventional (Styrofoam box) or automated system in Mini-Digitcool ZH 400. Seminal samples were evaluated after thawing for sperm motion parameters by CASA and plasma membrane integrity by epifluorescence microscopy.

Source: Journal of Equine Veterinary Science - October 24, 2018 Category: Veterinary Research Authors: Rosiara Rosaria Dias Maziero, Carlos Renato de Freitas Guaitolini, Priscilla Nascimento Guasti, Gabriel Augusto Monteiro, Ian Martin, Juliano Pianowski Marques da Silva, Andr é Maciel Crespilho, Frederico Ozanam Papa Source Type: research