Evaluation of DNA damage of mare granulosa cells before and after cryopreservation using a chromatin dispersion test

DNA fragmentation of granulosa cells might be related to developmental competence of the equine oocyte. Granulosa cells are commonly stored before DNA fragmentation assessment, but the effect of preservation methods on this parameter remains unexplored. The aim of this study was to evaluate whether or not cryopreservation of granulosa cells affects the DNA damage. Equine oocytes were recovered from post mortem ovaries of 5 mares. Granulosa cells were washed by centrifugation and then analysed (control) or stored in cryovials following four different protocols: P1= directly plunged in liquid nitrogen (LN2) and then stored at -80 ºC; P2=LN2/-80ºC adding cryoprotectants (7.5% ethylene glycol + 7.5% dimethyl sulfoxide); P3=-80ºC; P4=-80ºC+ cryoprotectants.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Tags: Short Communication Source Type: research