Unexpected decrease in in vivo binding of [3H]QNB in the mouse cerebral cortex in the developing brain - a comparison with [11C]NMPB

Significant discrepancies between in vitro and in vivo binding of the muscarinic receptor ligand - 3H-labeled Quinuclidinyl Benzylate (QNB) - have been well documented. Discernable in vivo cerebellar [3H]QNB binding has been observed in mouse brain, despite the maximum number of binding sites (Bmax) being low. In order to understand this unique in vivo binding phenomenon, the binding of two muscarinic receptor ligands -[3H]QNB and N-[11C]methylpiperidyl Benzylate ([11C]NMPB) - were compared in vivo and in vitro in 3- and 8-week-old mice.

Source: Nuclear Medicine and Biology - October 16, 2018 Category: Nuclear Medicine Authors: Osamu Inoue, Toshiyuki Sato, Kaoru Kobayashi, Antony Gee, Miho Shukuri, Ming-Rong Zhang Source Type: research