γ-Oryzanol supplementation modifies the inflammatory and oxidative response in fulminant hepatic failure in mice

Publication date: Available online 12 October 2018Source: PharmaNutritionAuthor(s): Marcelo Gomes de Gomes, Franciele Donato, Leandro Cattelan Souza, André Rossito Goes, Carlos Borges Filho, Lucian Del Fabbro, Matheus Chimelo Bianchini, Waseem Hassan, Silvana Peterini Boeira, Robson Luiz Puntel, Cristiano Ricardo JesseAbstractThe lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced hepatotoxicity is a classical model to elucidate the mechanisms and explore potential therapeutic drugs for fulminant hepatic failure (FHF). γ-Oryzanol (γ-ORY) has been showed to possess both antioxidant and anti-inflammatory properties in several pathological models. However, it remains unclear whether γ-ORY could protect against LPS/D-GalN-induced hepatotoxicity. Thus, the aim of the current study was to evaluate the effects of γ-ORY supplementation (for 7 consecutive days) in LPS/D-GalN-induced FHF in mice. One hour after the last supplementation with γ-ORY, the animals received LPS/D-GalN (50 and 500 mg/kg, respectively; i.p.). Twenty-four hours after LPS/D-GalN administration, biochemical assays were performed. LPS/D-GalN administration caused severe histological damage in the liver, such as vascular congestion and megalocytosis, as well as elicited hepatic failure. LPS/D-GalN treatment also caused alterations in liver function, increased levels of oxidative stress and inflammatory markers. In contrast, γ-ORY supplementation protected against histological, oxidative and infla...
Source: PharmaNutrition - Category: Nutrition Source Type: research