Freezing, Vitrification And Freeze-Drying Of Equine Spermatozoa: Impact On Mitochondrial Membrane Potential, Lipid Peroxidation And Dna Integrity

Maintaining the integrity of equine sperm subjected to preservation protocols is essential for the successful development of assisted reproduction procedures. The aim of this study was to assess the mitochondrial membrane potential, lipid peroxidation and DNA integrity of equine sperm subjected to freezing, vitrification and freeze-drying. Eight ejaculates obtained from four Colombian Creole horses were subjected to programmable freezing, vitrification and freeze-drying. After thawing or rehydration, sperm motility and kinetics were assessed through a CASA system.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research