Can sperm selection, inseminating dose and artificial insemination technique influence endometrial inflammatory response in mares?

The aim of this study was to investigate the influence of sperm concentration, sperm quality and semen deposition site on uterine inflammatory response in mares. Twenty-five mares with healthy reproductive tracts were used for artificial inseminations (AI) using frozen-thawed semen and divided into 5 experimental groups: Control - insemination with 800 ×106 total spermatozoa in the body of the uterus (n = 5); G2 - insemination with 40×106 total motile sperm (TMS) into uterine body (n = 5); G3 - insemination with 40×106 TMS at the uterotubal junction by hysteroscopy (HI) (n = 5); G4 - insemination with 40×106 TMS at the uterotubal junction by H I, with frozen-thawed semen selected by Percoll gradient (n = 5); G5 - insemination with 40×106 total motility sperm into uterine body, using frozen-thawed semen selected by Percoll gradient (n = 5).
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Tags: Original Research Source Type: research