New assay to diagnose and differentiate between Mycobacterium tuberculosis complex and nontuberculous mycobacteria

The purpose of the present study was to create a real-time PCR test system allowing simultaneous detection of nontuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) both in culture and sputum. NTM cultures (18 strains, 18 species), MTBC cultures (16 strains, 2 species) and non-mycobacterial microorganisms from the collection of the Central Research TB Institute (CTRI) were used for the preliminary evaluation of the test system. 301 NTM cultures from patients with mycobacteriosis were used to assess the sensitivity of the developed test system.

Source: Tuberculosis - October 8, 2018 Category: Respiratory Medicine Authors: Vera V. Ustinova, Tatiana G. Smirnova, Dmitry G. Sochivko, Dmitry A. Varlamov, Elena E. Larionova, Sofya N. Andreevskaya, Irina Yu. Andrievskaya, Ekaterina A. Kiseleva, Larisa N. Chernousova, Atadzhan Ergeshov Tags: Diagnostics Source Type: research