Combined Single-Straw Packaging of Cryopreserved Stallion Epididymal Sperm and Separated Homologous Seminal Plasma

The aim of the present study was the investigation of stallion epididymal sperm frozen with seminal plasma within the same straw separated by an air bubble before freezing. We hypothesized mixing both components after thawing improves the sperm quality compared to conventionally frozen-thawed epididymal sperm.Cryopreservation of epididymal sperm allows the preservation of spermatozoa in case of death or termination of the breeding carrier. However, these sperm lack contact with the seminal plasma, which plays an important role in the development of sperm functionality and the regulation of uterine inflammatory response following insemination.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Tags: Original Research Source Type: research