Clove (Syzygium aromaticum) and honey extracts significantly reduce inflammatory cytokines and liver function enzymes in experimental rats fed on carbon tetrachloride (CCl4)

Publication date: Available online 4 October 2018Source: Journal of Radiation Research and Applied SciencesAuthor(s): Mohamad T. Abdelrahman, Esther N. Maina, Hany A. ElshemyAbstractLiver is the key organ (detoxifier) that gets injured by reactive oxygen species (ROS) which deplete the body's stores of anti-oxidants. ROS is present in all chronic liver diseases leading to oxidative stress. Consumption of foods high in anti-oxidants replenishes and supplies the body with important anti-oxidants which break down ROS lowering inflammatory responses and preventing liver damage. In this work, clove and honey, which are known to be rich in anti-oxidants extracts were evaluated for their potential to breakdown ROS, Reactive nitrogen species (RNS) and lower oxidative stress in rats. Purified extracts were tested in vitro for ROS scavenging by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and in vivo using CCl4-induced liver injury model. A total of 50 experimental rats were assigned to 5 groups each consisting of 10 animals and treated as follows; 1. CCl4 group: fed on CCl4 in olive oil 2. honey group: fed CCl4 then honey extract, 3. clove group: CCl4 then clove extract, 4. double negative control group: rats fed on standard diet and 5 Negative control: fed olive oil, At 10 weeks, liver histopathology was done and sera collected for liver and kidney function tests. CCl4 -only treated rats showed fibroblastic cells proliferation and inflammatory cells infiltration which was absent in the contr...
Source: Journal of Radiation Research and Applied Sciences - Category: Physics Source Type: research