Exploiting a conjugative CRISPR/Cas9 system to eliminate plasmid harboring mcr-1 gene from Escherichia coli

The development of antibiotics has saved countless lives, however, over the past several decades, the intensive use of broad-spectrum antibiotics in medicine and animal agriculture has led to rapid bacterial evolution and the emergence of drug-resistant bacteria [1, 2]. Polymyxin, is a group of cationic polypeptide antibiotics commonly used in clinical veterinary to treat Gram-negative bacterial infections [3] and used in humans in limited situations as a last-line option for treating extensively antibiotic-resistant Gram-negative bacterial infections.

Source: International Journal of Antimicrobial Agents - September 26, 2018 Category: Drugs & Pharmacology Authors: Haisi Dong, Hua Xiang, Dan Mu, Dacheng Wang, Tiedong Wang Source Type: research