Do lipoxygenases occur in viruses?: Expression and characterization of a viral lipoxygenase-like protein did not provide evidence for the existence of functional viral lipoxygenases

Publication date: Available online 4 October 2018Source: Prostaglandins, Leukotrienes and Essential Fatty AcidsAuthor(s): Tatjana Gehring, Dagmar Heydeck, Agathe Niewienda, Katharina Janek, Hartmut KuhnAbstractLipoxygenases are lipid peroxidizing enzymes, which frequently occur in higher plants and animals. In bacteria, these enzymes are rare and have been introduced via horizontal gene transfer. Since viruses function as horizontal gene transfer vectors and since lipoxygenases may be helpful for releasing assembled virus particles from host cells we explored whether these enzymes may actually occur in viruses. For this purpose we developed a four-step in silico screening strategy and searching the publically available viral genomes for lipoxygenase-like sequences we detected a single functional gene in the genome of a mimivirus infecting Acantamoeba polyphaga. The primary structure of this protein involved two putative metal ligand clusters but the recombinant enzyme did neither contain iron nor manganese. Most importantly, it did not exhibit lipoxygenase activity. These data suggests that this viral lipoxygenase-like sequence does not encode a functional lipoxygenase and that these enzymes do not occur in viruses.Graphical abstract

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - October 4, 2018 Category: Lipidology Source Type: research