Identification and Characterization of Phospholipids with Very Long Chain Fatty Acids in Brewer ’s Yeast

AbstractYeast lipids and fatty acids (FA) were analyzed inSaccharomyces pastorianus from seven breweries and in the dietary yeast supplement Pangamin. GC –MS identified more than 30 FA, half of which were very-long chain fatty acids (VLCFA) with hydrocarbon chain lengths of ≥22 C atoms. Positional isomers ω-9 and ω-7 were identified in FA with C18–C28 even-numbered alkyl chains. The most abundant ω-7 isomer wascis-vaccenic acid. The structure of monounsaturated FA was proved by dimethyl disulfide adducts (position of double bonds andcis geometric configuration) and by GC –MS of pyridyl carbinol esters. Ultra-high performance liquid chromatography-tandem mass spectrometry with negative electrospray ionization identified the phospholipids phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine, with more than 150 molecular species. Wild-type unmutated brewer’s yeast strains conventionally used for the manufacture of food supplements were found to contain VLCFA.

Source: Lipids - December 1, 2017 Category: Lipidology Source Type: research