UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences

Publication date: Available online 26 September 2018Source: International Journal for Parasitology: Drugs and Drug ResistanceAuthor(s): Petra Matoušková, Lenka Lecová, Roz Laing, Diana Dimunová, Heiko Vogel, Lucie Raisová Stuchlíková, Linh Thuy Nguyen, Pavlína Kellerová, Ivan Vokřál, Jiří Lamka, Barbora Szotáková, Marián Várady, Lenka SkálováAbstractUDP-glycosyltransferases (UGT), catalysing conjugation of UDP-activated sugar donors to small lipophilic chemicals, are widespread in living organisms from bacteria to fungi, plant, or animals. The progress of genome sequencing has enabled an assessment of the UGT multigene family in Haemonchus contortus (family Trichostrongylidae, Nematoda), a hematophagous gastrointestinal parasite of small ruminants. Here we report 32 putative UGT genes divided into 15 UGT families. Phylogenetic analysis in comparison with UGTs from Caenorhabditis elegans, a free-living model nematode, revealed several single member homologues, a lack of the dramatic gene expansion seen in C. elegans, but also several families (UGT365, UGT366, UGT368) expanded in H. contortus only. The assessment of constitutive UGT mRNA expression in H. contortus adults identified significant differences between females and males. In addition, we compared the expression of selected UGTs in the drug-sensitive ISE strain to two benzimidazole-resistant strains, IRE and WR, with different genetic backgrounds. Constitutive expression of UGT368B2 was significantly...
Source: International Journal for Parasitology: Drugs and Drug Resistance - Category: Parasitology Source Type: research