Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity

Conclusion 1) Unnecessary incubation of spermatozoa prior to artificial insemination or in vitro fertilization, should be avoided, since sperm DNA longevity is significantly reduced after ex vivo sperm handling and 2) Although sperm selection by DCG significantly reduces the baseline levels of SDF of sperm in Fraction 3, sperm DNA longevity in this fraction was ultimately lower following 24 h incubation when compared to sperm recovered from non-centrifuged NSS.
Source: Asian Pacific Journal of Reproduction - Category: Reproduction Medicine Source Type: research