Effect of the Bowl Structure in an Automated Cell-Isolation Device on Stromal Vascular Fraction's Isolation Yield

In this study, we evaluated the efficiency and the reliability of a new automated SVF isolation system in which the bowl was designed in the shape of a radial protrusion at each angle (a top-type bowl). The viability and yield of cells and the residual collagenase activities of SVFs obtained in a top-type bowl were compared with the SVFs obtained in a conventional bowl. We achieved a significantly higher yield of cells and decreased residual collagenase activity in the SVFs obtained from a top-type bowl (18.0  × 105 cells/mL of fat) compared to a conventional bowl (2.3  × 105 cells/mL). There was no significant difference in the cell viability between the two groups. These results suggest that the automated SVF isolation system with an improved bowl structure will potentially yield higher numbers of nucleated cells and decreased residual collagenase activity compared to conventional automated systems in cell-based clinical trials.
Source: Journal of Medical Devices, Transactions of the ASME - Category: Medical Devices Source Type: research