Angiotensin II upregulates CYP4A isoform expression in the rat kidney through angiotensin II type 1 receptor

Publication date: Available online 15 September 2018Source: Prostaglandins & Other Lipid MediatorsAuthor(s): Rong Rong, Wanting Wang, Yoshikazu Muroya, Gaizun Hu, Takahiro Miura, Yoshiko Ogawa, Masahiro Kohzuki, Osamu ItoAbstractAngiotensin II (AngII) stimulates the renal production and release of 20-hydroxyeicosatetraenoic acids (20-HETE), which is a major metabolite of arachidonic acid catalyzed by CYP4 A isoforms. However, the effects of AngII on CYP4 A isoform expression in the kidney and its mechanism remains unclear. To clarify the regulation of CYP4 A isoform expression by AngII, we examined the chronic effects of AngII and AngII type 1 receptor (AT1-R) blockade on CYP4 A isoform expression. Sprague-Dawley rats were infused with vehicle or AngII for 1 week, and the AngII-infused rats were also treated with or without the AT1-R blocker, candesartan. AngII increased CYP4 A isoform protein expression in the renal cortex (CO) and outer medulla (OM) in a dose-dependent manner, and candesartan inhibited the AngII-increased CYP4 A expression in a dose-dependent manner. AngII increased the CYP4 A isoform mRNA expression in the CO and OM, and candesartan inhibited AngII-increased CYP4 A isoform mRNA expression. These results indicated that AngII chronically increased the CYP4 A isoform expression in the rat kidney. The AngII-induced CYP4 A isoform expression was mediated by AT1-R.
Source: Prostaglandins and Other Lipid Mediators - Category: Lipidology Source Type: research