PCR assays for detection of human astroviruses: in silico evaluation and design, and in vitro application to samples collected from patients in the Netherlands

Following the discovery of astrovirus in human stool [1], diverse astroviruses were described in humans and other hosts. They were classified in the family Astroviridae that is composed of two genera, Mamastrovirus and Avastrovirus [2]. Human astroviruses (HAstV) belong to the genus Mamastrovirus that includes four established species: Mamastrovirus 1 (MAstV1; classic HAstV or HAstV-C, types 1-8), Mamastrovirus 6 (MAstV6; known as HAstV-MLB, types 1-3), and closely related Mamastrovirus 8 and Mamastrovirus 9 (MAstV8 and MAstV9; both known as HAstV-VA/HMO, types 1-5 and A-C, respectively), and HAstV-BF34 a prototype of a putative separate species [3,4].

Source: Journal of Clinical Virology - September 13, 2018 Category: Virology Authors: R.H.T. Nijhuis, I.A. Sidorov, P.K. Chung, E. Wessels, A.A Gulyaeva, J.J. de Vries, E.C.J. Claas, A.E. Gorbalenya Source Type: research