Genotypic variation in Acmella paniculata across different phytogeographical ranges of Northeast India inferred through ISSR & SCoT based markers

Publication date: Available online 7 September 2018Source: Journal of Applied Research on Medicinal and Aromatic PlantsAuthor(s): Debmalya Das Gupta, Pallabi K. Hui, Hui TagAbstractPresent study is intended to find out genotypic variation in Acmella paniculata distributed across larger phytogeographical ranges and along different altitudinal gradient of Northeast India using ISSR and SCoT markers. Acmella paniculata (Wall. ex DC.) R.K. Jansen (family Asteraceae) is an important medicinal herb distributed worldwide in both tropical and subtropical regions. A total of 98 individuals of A. paniculata belonging to 10 natural populations spots were sampled from different altitudinal gradient of 03 major phytogeographical ranges of Northeast India, and the genotypic variation within and among the populations were inferred using ISSR and SCoT markers. In the ISSR marker based study, a total of 429 loci were obtained in which 178 loci were monomorphic and 251 loci were polymorphic showing 58% polymorphism. In the SCoT based marker study, a total of 791 amplification products were scored of which 588 were polymorphic, exhibiting 74% polymorphism. The PIC value of ISSR and SCoT marker system were 0.67 and 0.83 respectively. The Rp value of the ISSR primers were ranging from 4.120 to 7.22, whereas it was 5.24 to 9.20 for the SCoT primers. Jaccard’s coefficient of similarity were ranging from 0.1082 – 0.7325 in case of ISSR, whereas it was 0.1366 – 0.9253 for SCoT markers. GST valu...
Source: Journal of Applied Research on Medicinal and Aromatic Plants - Category: Complementary Medicine Source Type: research