Prevalence and sequence of aminoglycosides modifying enzymes genes among E.coli and Klebsiella species isolated from Egyptian hospitals

Publication date: Available online 4 September 2018Source: Journal of Radiation Research and Applied SciencesAuthor(s): Mervat Aly Mohamed Abo-State, Youssry El-Sayed Saleh, Hazem Mahmmoud GhareebAbstractThe WHO and CDC have expressed serious concern regarding the continued increase in the development of multidrug resistance among bacteria. Associated with the rise in antibiotic resistance is the lack of new antimicrobials. Bacteria have developed many ways by which they become resistant to antimicrobials among those are enzymes. Aminoglycosides play an important role in treatment of serious infections that threat human life caused by Gram-negative bacteria (GNB). In our study, clinical bacterial isolates(210) were collected from different Egyptian hospitals. Eight aminoglycoside antibiotics were used in the present study. The most prevalent pathogen were E. coli (36.66%),E.coli-ESBL(5.23%),Klebsiella spp. (25.33%), Klebsiella spp.–ESBL (1.90%),Pseudomonas spp.(17.61%), Acinetobacter spp. (8.09%),Proteus spp. (2.85%),Enterobacter spp.(1.42%) and (0.47%) for both of Citrobacter sp. and Morganella sp. The most efficiency aminoglycoside antibiotic was Amikin(AK) and the resistance pattern increased over the last years in Egypt. The results of AME-genes indicated that non of aac(3′)-Ia and Rmt(55) genes were detected in any of the thirty Klebsiella spp. and E. coli of Egyptian isolates. The most prevalent AME-genes were aac(3′)-IIa(40%),aac(6′)-Ib (30%) followed by aph(3â...
Source: Journal of Radiation Research and Applied Sciences - Category: Physics Source Type: research