PRMT5 inhibition promotes osteogenic differentiation of mesenchymal stromal cells and represses basal interferon stimulated gene expression

Protein arginine methyltransferases (PRMTs) catalyze symmetric and asymmetric methylation on arginine residues of multiple protein targets including histones and have essential roles in organismal development and disease. PRMT5 mediates symmetric di-methylation (sDMA) of arginine 2 (H3R2me2) and arginine 8 on histone 3 (H3R8me2), arginine 3 on histones 2A and 4 (H2A/H4R3me2) as well as several non-histone substrates like Sm proteins. Here, we found that selective inhibition of Prmt5 in mesenchymal stromal cells (MSCs) led to a reduction in colony forming units (CFUs) and increased osteoblast differentiation.

Source: Bone - September 3, 2018 Category: Orthopaedics Authors: Satya K. Kota, Coco Roening, Nehal Patel, Savithri B. Kota, Roland Baron Tags: Full Length Article Source Type: research