In situ characterization of change in superficial organic components of thermoacidophilic archaeon Acidianus manzaensis YN-25

Publication date: Available online 1 September 2018Source: Research in MicrobiologyAuthor(s): Li-Zhu Liu, Zhen-Yuan Nie, Yi Yang, Xuan Pan, Xu Xia, Yu-Hang Zhou, Jin-Lan Xia, Li-juan Zhang, Xiang-jun Zhen, Hong-ying YangAbstractFor the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2, CuFeS2, S0, FeSO4). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount. XANES results showed clear associations between the energy substrates and the changes in organic composition in terms of typical function groups (-C=O, -C-O and -C-N). The chalcopyrite- and pyrite-acclimated cells contained higher proportion of proteins but less proportion of polysaccharides than the S0-acclimated cells. The FeSO4-acclimated cells contained the highest proportion of proteins, while the S0-acclimated cells contained more lipids and polysaccharides. The results of linear-combination and peak fitting of the K-edge XANES for the extracellular superficial organic component C is consistent with the trend in comparison with the results of FTIR and spectrophotometric determination, but there ar...
Source: Research in Microbiology - Category: Microbiology Source Type: research