Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection

After ligand binding, receptor tyrosine kinases (RTKs) transmit intracellular signals involved in the regulation of various cell events and then attenuate signal transduction. Ubiquitination is a critical step involved in the downregulation of RTK signaling. Here, we describe how to immunodetect the ligand-induced ubiquitination and degradation of TrkA, an RTK, by immunoprecipitation and Western blotting.

Source: Springer protocols feed by Protein Science - October 17, 2014 Category: Biochemistry Source Type: news