Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1

FISH (fluorescent in situ hybridization) is a molecular cytogenetic method to detect large-scale genetic alterations in tissue and/or cells. Numerical aberrations (deletions and amplifications) and structural aberrations (translocations and fusions) are detectable. Probes bind complementary to the DNA strand of the region of interest. Subsequently, the probes are detected via fluorochromes and appear as colored dots that can be assessed under the fluorescence microscope.
Source: Springer protocols feed by Protein Science - Category: Biochemistry Source Type: news