Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells

We describe optical tracking of quantum dot (QD)-labeled single receptors using the total internal reflection fluorescence microscopy (TIRFM), and initial steps of data analysis to identify the time-dependent variation of single-receptor diffusion, which can be widely applied to studying activation of various cell surface receptors.

Source: Springer protocols feed by Protein Science - October 17, 2014 Category: Biochemistry Source Type: news