Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

Immunoblotting for phosphorylated forms of receptor tyrosine kinases (RTKs) has been the mainstay of investigations on RTK signaling for the past two decades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling networks involving RTKs.

Source: Springer protocols feed by Protein Science - October 17, 2014 Category: Biochemistry Source Type: news