Evaluation of antioxidant properties of different extracts of Chaetomium cupreum SS02

Publication date: Available online 24 August 2018Source: Bulletin of Faculty of Pharmacy, Cairo UniversityAuthor(s): Nazir Ahmad Wani, Sharmila TirumaleAbstractTo evaluate the antioxidant properties of different extracts (chloroform, ethyl acetate, n-butanol and methanol extracts) of fungus C. cupreum SS02. The antioxidant properties were evaluated by using five antioxidant methods, metal chelating assay, 2,2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS), hydroxyl radical (HO) scavenging assay, superoxide anion (O2) radical scavenging assay, and nitric oxide (NO) scavenging assay. Methanol extract of C. cupreum showed highest metal chelating activity (63.61 ± 0.07 mg EDTAE/g DW), followed by chloroform extract (57.25 ± 0.12 mg E DTAE/g DW) and n-butanol extract (27.33 ± 0.07 mg E DTAE/g DW) whereas ethyl acetate extract showed least metal chelating activity (18.35 ± 0.07 mg E DTAE/g DW) at 50 µg/ml. In ABTS assay, n-butanol extract showed highest ABTS inhibition activity (19.91 ± 0.14 μmol RE/g DW), followed by ethyl acetate extract (13.07 ± 0.59 μmol RE/g DW) and chloroform extract (13.07 ± 0.82 μmol RE/g DW) whereas methanol extract showed the lowest activity (11.65 ± 0.16 μmol RE/g DW at 50 µg/ml). In hydroxyl radical scavenging assay, n-butanol extract showed highest scavenging activity (31.95 ± 0.21 mg RE/g DW), followed by ethyl acetate extract (28.19 ± 0.21 mg RE/g DW), methanol ...
Source: Bulletin of Faculty of Pharmacy, Cairo University - Category: Drugs & Pharmacology Source Type: research