A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging

We describe a kinetic multiplex assay incorporating the CellPlayerTM NucLight Red reagent to measure proliferation and the CellPlayerTM Caspase-3/7 reagent to measure apoptosis using the two-color, live-content imaging platform, IncuCyteTM ZOOM. High-definition phase-contrast images provide an additional qualitative validation of cell death based on morphological characteristics. The kinetic data generated using this strategy can be used to derive informed pharmacology measurements to screen potential cancer therapeutics.

Source: Springer protocols feed by Cancer Research - October 15, 2014 Category: Cancer & Oncology Source Type: news