E3-ubiquitin ligase NEDD4 enhances bone formation by removing TGF β1-induced pSMAD1 in immature osteoblast

Neural precursor cell expressed developmentally downregulated protein 4 (NEDD4) is an E3 ubiquitin ligase that regulates animal growth and development. To investigate the role of NEDD4 in skeletogenesis in vivo, we established immature osteoblast-specific 2.3-kb Collagen Type I Alpha 1 chain (Col1 α1) promoter-driven Nedd4 transgenic (Nedd4-TG, Col1α1-Nedd4Tg/+) mice and conditional knockout (Nedd4-cKO, Col1α1-Cre;Nedd4fl/fl) mice. The Nedd4-TG mice displayed enhanced bone mass accrual and upregulated gene expression of osteogenic markers in bone.

Source: Bone - August 17, 2018 Category: Orthopaedics Authors: Seon-Ae Jeon, Ji-Hyun Lee, Dong Wook Kim, Je-Yoel Cho Tags: Full Length Article Source Type: research