Comparative Study on Manuka and Indonesian Honeys to Support the Application of Plasma Jet during Proliferative Phase on Wound Healing

Publication date: Available online 11 August 2018Source: Clinical Plasma MedicineAuthor(s): Eka Sakti Wahyuningtyas, Arya Iswara, Yunita Sari, Sodiq Kamal, Budi Santosa, Tatsuo Ishijima, Toshio Nakatani, Indri Kartika Putri, N NasruddinAbstractA comparative investigation was conducted to determine the effectiveness of Manuka and Indonesian honeys to support the application of plasma jet treatment during the proliferative phase of cutaneous wound healing in 8-week-old, BALB/c male mice. The effect of honey containing different concentrations to reactive oxygen and nitrogen species (RONS) produced by plasma jet in liquid medium using H2O2 as the indicator was conducted using chemical-enzymatic method. Plasma jet treatment was applied perpendicularly to wounds through holes punched in multiple micro-well dressings (MMD) using direct contact. Mice were divided into 4 groups: Hydrocolloid dressing alone (Control group or C), plasma application followed by hydrocolloid dressing (PH), plasma application followed by treatment with Indonesian honey (PI), and plasma application followed by treatment with Manuka honey (PM). Two full-thickness acute wounds were created on both sides of the mouse dorsum using a disposable biopsy punch. The wounds of the control group were covered with a hydrocolloid dressing (HD), whereas wounds in the other groups were covered with a HD from days 0 to 3, treated with plasma followed by 0.1 mL of the relevant honey or HD from days 4 to 7 post-wounding, an...
Source: Clinical Plasma Medicine - Category: Research Source Type: research