A stable method for routine analysis of oxylipins from dried blood spots using ultra-high performance liquid chromatography–tandem mass spectrometry

In this study, we report the development and validation of a novel system for the quantitation of 21 individual oxylipins from a dried blood spot (DBS) using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) and stable isotope dilution analysis. Linearity and precision of the method were determined and the stabilities of the 12 most abundant oxylipins were tested during 2 months of storage at room temperature, after being spiked into blood and prepared as DBS on PUFAcoatTM paper. Responses were linear across the concentration range analysed for all oxylipins (r2 values ranged from 0.953 – 0.998). Intra–day and inter–day variations were ≤ 16% for all oxylipins. Recovery of oxylipins from the DBS ranged from 80 – 115%. The 12 spiked oxylipins were stable for 2 months when stored as DBS at room temperature. Our method is reproducible and precise, and provides the opportunity to accurately quantitate these oxylipins in a small sample volume.Graphical Abstract

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - August 2, 2018 Category: Lipidology Source Type: research