The architecture of an Okazaki fragment-processing holoenzyme from the archaeon Sulfolobus solfataricus

DNA replication on the lagging strand occurs via the synthesis and maturation of Okazaki fragments. In archaea and eukaryotes the enzymatic activities required for this process are supplied by a replicative DNA polymerase, Flap Endonuclease 1 (Fen1) and DNA ligase 1. These factors interact with the sliding clamp PCNA providing a potential means of coordinating their sequential actions within a higher order assembly. In hyperthermophilic archaea of the Sulfolobus genus PCNA is a defined heterotrimeric assembly and each subunit interacts preferentially with specific client proteins. We have exploited this inherent asymmetry to assemble a PCNA/Polymerase/Fen1/Ligase complex on DNA and have visualized it by electron microscopy. Our studies reveal the structural basis of co-occupancy of a single PCNA ring by the three distinct client proteins.

Source: BJ Gene - October 9, 2014 Category: Biochemistry Authors: G Cannone, Y Xu, T R. Beattie, S D Bell, L Spagnolo Tags: BJ Gene Source Type: research