Trypanosoma cruzi epimastigotes store cholesteryl esters in lipid droplets after cholesterol endocytosis

Publication date: September 2018Source: Molecular and Biochemical Parasitology, Volume 224Author(s): Miria G. Pereira, Gonzalo Visbal, Tatiana F.R. Costa, Susana Frases, Wanderley de Souza, Geórgia Atella, Narcisa Cunha-e-SilvaAbstractThe Chagas disease agent Trypanosoma cruzi proliferates in the insect vector as highly endocytic epimastigotes that store nutrients, including lipids in reservosomes (lysosome related compartments). Although nutrient storage is important for epimastigote transformation into infective metacyclics, the epimastigote lipid droplets (LDs) remain uncharacterized. Here, we characterized the epimastigote LDs and examined their relationship with the endocytic pathway. Fluorescence microscopy using BODIPY showed that LDs have high neutral lipid content and harbor Rab18, differently from other lipid-rich organelles (such as reservosomes). Using transmission electron microscopy (TEM), we observed a close relationship between LDs and the endoplasmic reticulum, mitochondria and glycosomes. We developed a reproducible protocol to isolate LDs, and showed (by HTPLC and GC/MS analyses) that they have 89% neutral lipids and 11% phospholipids, which are likely to form the LD monolayer seen by TEM. The LD neutral lipids were mostly sterols, although triacylglycerol, diacylglycerol, monoacylglycerol and free fatty acids (FFA) were also found. Endocytosis of 3H-labeled cholesterol-BSA showed that internalized cholesterol is stored in LDs mostly in the cholesteryl est...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research