Diagnostic accuracy of digital RNA quantification versus real-time PCR for the detection of respiratory syncytial virus in nasopharyngeal aspirates from children with acute respiratory infection

Respiratory syncytial virus (RSV) is one of the most common etiological agents of acute respiratory infections (ARI) among children such as bronchiolitis and pneumonia [1,2]. RSV can be divided into two subgroups (A and B) that commonly produce annual epidemics characterized by the circulation of several genotypic strains [3]. The seasonality of RSV-A and RSV-B can be markedly different [4]. Virus-specific molecular assays such as real-time polymerase chain reaction (RT-PCR) are now considered the gold standard in the diagnosis of viral respiratory tract infections.

Source: Journal of Clinical Virology - July 15, 2018 Category: Virology Authors: Maiara L. Bouzas, Juliana R. Oliveira, Artur Queiroz, Kiyoshi F. Fukutani, Aldina Barral, Annabel Rector, Elke Wollants, Els Keyaerts, Winke Van der Gucht, Marc Van Ranst, Kurt Beuselinck, Camila I. de Oliveira, Johan Van Weyenbergh, Cristiana M. Nascimen Source Type: research