A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Publication date: July 2018Source: Journal of Food and Drug Analysis, Volume 26, Issue 3Author(s): Li-Ying Hsieh, Hsiu-Hui Chan, Ping-Chung Kuo, Hsin-Yi Hung, Yue-Chiun Li, Chao-Lin Kuo, Yong Peng, Zhong-Zhen Zhao, Daih-Huang Kuo, I-Wen Sun, Tian-Shung WuAbstractLycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.Graphical abstract
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research